ABSTRACT
PURPOSE: The purpose of this study was to explore the influence in a planned pregnancy of alcohol related family variables, knowledge and recognition of the effects of alcohol use during pregnancy on actual alcohol use during pregnancy. METHODS: The participants were 284 women who had experienced a pregnancy at some time in their lives. The data were collected from December 2011 to January 2012 and the method of data collection was self-report questionnaires. The instruments for this study were AUDIT-K, Knowledge of alcohol use during pregnancy, and Recognition of alcohol use during pregnancy. RESULTS: There were higher incidences of alcohol use during pregnancy when alcohol consumption was a problem, when there was a family member with an alcohol problem, or after having had an artificial abortion. There was no correlation in alcohol use during pregnancy with knowledge, but a correlation with recognition was found. CONCLUSION: The results of this study suggest that the main factor in alcohol use during pregnancy is recognition of the effects of alcohol use during pregnancy.
Subject(s)
Female , Humans , Pregnancy , Alcohol Drinking , Data Collection , Family Planning Services , Incidence , Surveys and QuestionnairesABSTRACT
Subject(s)
Animals , Rats , Absorption , Alveolar Process , Bone Regeneration , Collagen , Collagen Type I , Giant Cells , Lymphocytes , Membranes , Osteogenesis , Rats, Sprague-Dawley , TransplantsABSTRACT
PURPOSE: Collagen is the principal structural biomolecule in cartilage extracellular matrix, which makes it a logical target for cartilage engineering. In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) for cartilage regeneration. METHODS: Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four types of scaffolds(n=4) were inserted. The types included DHT cross-linked(Group 1), DHT and EDC cross- linked(Group 2), CS added DHT cross-linked(Group 3), and CS added DHT and EDC cross-linked(Group 4). Histomorphometric analysis and cartilage-specific gene expression of the reconstructed tissues were evaluated respectively 4, 8, and 12 weeks after implantation. RESULTS: The largest quantity of regenerated cartilage was found in DHT cross-linked groups 1 and 3 in the 8th week and then decreased in the 12th week, while calcification increased. Calcification was observed from the 8th week and the area increased in the 12th week. Group 4 was treated with EDC cross-linking and CS, and the matrix did not degrade in the 12th week. Cartilage-specific type II collagen mRNA expression increased with time in all groups. CONCLUSION: CS did not increase chondrogenesis in all groups. EDC cross-linking may prevent chondrocyte infiltration from the perichondrium into the collagen scaffold.
Subject(s)
Rabbits , Cartilage , Chondrocytes , Chondrogenesis , Chondroitin Sulfates , Collagen , Collagen Type I , Collagen Type II , Ear , Ear Cartilage , Extracellular Matrix , Gene Expression , Logic , Regeneration , RNA, MessengerABSTRACT
<0.05). 4. Histologic examination revealed that AM protected leukocyte infiltration and epithelial migration was nearly completed at 4 weeks. Terudermis(R) group showed mild neutrophil infiltration until 2 weeks and completion of epithelization at 4 weeks. Control group showed massive leukocyte infiltration until 4 weeks. 5. Microvessels were increased sharply at 1 week and control group at 1 and 4 week showed significant differences with Terudermis(R) group of same interval(p<0.05) but no differences were found with AM group(p<0.05). CONCLUSION: EGF and EGF-R were well preserved in freeze-dried AM. AM attached to collagen acted as excellent biologic dressing which had similar effect with Terudermis(R). AM showed anti-inflammatory action and healing was completed at 4 weeks after full-thickness skin defect.
Subject(s)
Animals , Rats , Amnion , Bandages , Biological Dressings , Collagen , Epidermal Growth Factor , Leukocytes , Microvessels , Neutrophil Infiltration , Skin , Wound Healing , Wounds and InjuriesABSTRACT